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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
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Biochemie
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JoVE Journal Biochemie
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

2,877 Views

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08:30 min

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February 02, 2021

DOI:

10.3791/62201-v

DOI:
10.3791/62201-v

•

08:30 min
February 02, 2021

•

1 Views
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1Department of Cell Biology,University of Texas Southwestern Medical Center

Summary

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Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

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Metabolic LabelingThin Layer ChromatographyLipid MetabolismSaccharomyces CerevisiaeYeast CultureRadiolabelingQuenching BufferCentrifugationLipid SynthesisEnzyme Regulation

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Rogers, S., Henne, W. M. Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography. J. Vis. Exp. (168), e62201, doi:10.3791/62201 (2021).

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