Dergi
/
/
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

DOI:

08:30 min

February 02, 2021

,

Bölümler

  • 00:04Introduction
  • 00:30Growth and Labelling of Yeast Cells
  • 02:55Isolation of Total Lipids from Yeast
  • 04:42Thin Layer Chromatography and Quantification of Results
  • 07:09Results: 14C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
  • 08:03Conclusion

Özet

Otomatik Çeviri

Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

İlgili Videolar

Read Article