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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
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生物化学
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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

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08:30 min

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February 02, 2021

DOI:

10.3791/62201-v

DOI:
10.3791/62201-v

•

08:30 min
February 02, 2021

•

1 Views
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1Department of Cell Biology,University of Texas Southwestern Medical Center

Summary

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Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

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Metabolic LabelingThin Layer ChromatographyLipid MetabolismSaccharomyces CerevisiaeYeast CultureRadiolabelingQuenching BufferCentrifugationLipid SynthesisEnzyme Regulation

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Rogers, S., Henne, W. M. Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography. J. Vis. Exp. (168), e62201, doi:10.3791/62201 (2021).

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