Analysis of Neutral Lipid Synthesis in <em>Saccharomyces cerevisiae</em> by Metabolic Labeling and Thin Layer Chromatography

Sean Rogers; W. Mike Henne

doi:10.3791/62201

Journal

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Biochimie

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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

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Biochimie

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Journal JoVE Biochimie

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

2,877 Views

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08:30 min

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February 02, 2021

DOI:

10.3791/62201-v

DOI:

10.3791/62201-v

•

08:30 min

February 02, 2021

•

1 Views

Sean Rogers¹, W. Mike Henne¹

¹Department of Cell Biology,University of Texas Southwestern Medical Center

Summary

Here, a protocol is presented for the metabolic labeling of yeast with ¹⁴C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

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Metabolic Labeling Thin Layer Chromatography Lipid Metabolism Saccharomyces Cerevisiae Yeast Culture Radiolabeling Quenching Buffer Centrifugation Lipid Synthesis Enzyme Regulation

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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

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