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通过代谢标记和薄层色谱分析糖浆中中性脂质合成

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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

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通过代谢标记和薄层色谱分析糖浆中中性脂质合成

DOI:

10.3791/62201-v

•

08:30 min

•

February 02, 2021

•

Sean Rogers, W. Mike Henne

¹Department of Cell Biology,University of Texas Southwestern Medical Center

Chapters

00:04Introduction
00:30Growth and Labelling of Yeast Cells
02:55Isolation of Total Lipids from Yeast
04:42Thin Layer Chromatography and Quantification of Results
07:09Results: ¹⁴C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
08:03Conclusion

Summary

자동 번역

在这里，为酵母的代谢标记提供 ¹⁴个C-醋酸，这与薄层色谱相结合，用于中性脂质的分离。

Tags

Metabolic Labeling Thin Layer Chromatography Lipid Metabolism Saccharomyces Cerevisiae Yeast Culture Radiolabeling Quenching Buffer Centrifugation Lipid Synthesis Enzyme Regulation

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