Journal
/
신경과학
/
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

7,126 Views

•

10:45 min

•

June 14, 2020

DOI:

10.3791/61046-v

DOI:
10.3791/61046-v

•

10:45 min
June 14, 2020

•

7 Views
, , ,
1Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física,Universidad de Valencia

Summary

Automatically generated

Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.

Explore More Videos

Double In Utero ElectroporationCorticogenesisNeuron CellsNeuronal DisordersAutismSchizophreniaLateral VentriclesFast Green DyePlasmid DNAAnesthetized Pregnant MouseRazor70% EthanolIodine WipesRing ForcepsMouth Controlled Aspirator TubeLateral VentricleSquare-wave ElectroporaterCortical HemLateral Cortex

Read Article

Cite this Article

Mateos-White, I., Fabra-Beser, J., de Agustín-Durán, D., Gil-Sanz, C. Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations. J. Vis. Exp. (160), e61046, doi:10.3791/61046 (2020).

Download .ris file

Copy

Share Video

.

Copy