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Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
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Neuroscience
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JoVE Journal Neuroscience
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

English

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7,126 Views

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10:45 min

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June 14, 2020

DOI:

10.3791/61046-v

DOI:
10.3791/61046-v

•

10:45 min
June 14, 2020

•

7 Views
, , ,
1Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física,Universidad de Valencia

Summary

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Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.

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Mateos-White, I., Fabra-Beser, J., de Agustín-Durán, D., Gil-Sanz, C. Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations. J. Vis. Exp. (160), e61046, doi:10.3791/61046 (2020).

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