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Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
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Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
Kapitel
- 00:04Introduction
- 01:09First In Utero Electroporation Surgery
- 03:20Second In Utero Electroporation
- 04:18Tissue Harvesting and Sectioning: Strategy A
- 06:09Tissue Harvesting and Sectioning: Strategy B
- 07:24Confocal Fluorescence Imaging and Analysis
- 08:34Results: Representative Double Electroporation-Facilitated Cell Interaction Visualization Strategies
- 09:55Conclusion
Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.
Tags
Double In Utero ElectroporationCorticogenesisNeuron CellsNeuronal DisordersAutismSchizophreniaLateral VentriclesFast Green DyePlasmid DNAAnesthetized Pregnant MouseRazor70% EthanolIodine WipesRing ForcepsMouth Controlled Aspirator TubeLateral VentricleSquare-wave ElectroporaterCortical HemLateral Cortex
