/
/
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
7,126 Views
•
10:45 min
•
June 14, 2020
DOI:
Summary
Automatically generated
Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.
Explore More Videos
Double In Utero ElectroporationCorticogenesisNeuron CellsNeuronal DisordersAutismSchizophreniaLateral VentriclesFast Green DyePlasmid DNAAnesthetized Pregnant MouseRazor70% EthanolIodine WipesRing ForcepsMouth Controlled Aspirator TubeLateral VentricleSquare-wave ElectroporaterCortical HemLateral Cortex
Read Article
Cite this Article
Mateos-White, I., Fabra-Beser, J., de Agustín-Durán, D., Gil-Sanz, C. Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations. J. Vis. Exp. (160), e61046, doi:10.3791/61046 (2020).
Copy