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Neuroscience

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JoVE Journal Neuroscience
Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations
 

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Article DOI: 10.3791/61046-v • 10:45 min • June 14th, 2020
June 14th, 2020 •
1, 1, 1, 1
1Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física, Universidad de Valencia

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Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.

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Double In Utero Electroporation Mammalian Corticogenesis Neuron Cells Cell Populations Interaction Developmental Ages Unperturbed Interactions Wiring Alteration Neuronal Disorders Autism Schizophrenia Neocortex Exocortico Areas Sepalium Cerebellum Injection Of DNA Lateral Ventricles Bright Light Source Surgery Preparation Fast Green Dye Plasmid DNA Solution Microcapillary Pipette Anesthetized Pregnant Mouse
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