Journal
/
발생학
/
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

5,830 Views

•

10:14 min

•

February 27, 2021

DOI:

10.3791/61034-v

DOI:
10.3791/61034-v

•

10:14 min
February 27, 2021

•

20 Views
, ,
1Departamento de Biologia,Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze,Universitá degli Studi di Milano

Summary

Automatically generated

This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.

Explore More Videos

Fluorescent ImmunolocalizationArabinogalactan ProteinsPectinsCell WallFixationVacuumDehydrationEmbeddingLR-white Resin

Read Article

Cite this Article

Costa, M., Pereira, A. M., Coimbra, S. Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues. J. Vis. Exp. (168), e61034, doi:10.3791/61034 (2021).

Download .ris file

Copy

Share Video

.

Copy