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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Bölümler
- 00:09Introduction
- 00:39Sample Fixation and Dehydration
- 02:17LR-White Resin Embedding
- 03:26Sample Trimming and Sectioning
- 05:12Immunolocalization
- 07:31Results: Subcellular Localization of Proteins and Pectins
- 09:32Conclusion
This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.
