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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
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発生生物学
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JoVE Journal 発生生物学
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

5,835 Views

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10:14 min

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February 27, 2021

DOI:

10.3791/61034-v

DOI:
10.3791/61034-v

•

10:14 min
February 27, 2021

•

20 Views
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1Departamento de Biologia,Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze,Universitá degli Studi di Milano

概要

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This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.

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Cite this Article

Costa, M., Pereira, A. M., Coimbra, S. Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues. J. Vis. Exp. (168), e61034, doi:10.3791/61034 (2021).

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