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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
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Biologie du développement
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Journal JoVE Biologie du développement
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

5,830 Views

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10:14 min

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February 27, 2021

DOI:

10.3791/61034-v

DOI:
10.3791/61034-v

•

10:14 min
February 27, 2021

•

20 Views
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1Departamento de Biologia,Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze,Universitá degli Studi di Milano

Summary

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This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.

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Fluorescent ImmunolocalizationArabinogalactan ProteinsPectinsCell WallFixationVacuumDehydrationEmbeddingLR-white Resin

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Cite this Article

Costa, M., Pereira, A. M., Coimbra, S. Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues. J. Vis. Exp. (168), e61034, doi:10.3791/61034 (2021).

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