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Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
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January 21, 2020
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A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.
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Bigdelou, P., Farnoud, A. M. Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore. J. Vis. Exp. (155), e60659, doi:10.3791/60659 (2020).
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