Journal
/
Biochimie
/
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

DOI:
10.3791/60659-v

•

09:15 min

•

January 21, 2020

•

,
1Biomedical Engineering Program,Ohio University, 2Department of Chemical and Biomolecular Engineering,Ohio University

Chapitres

  • 00:04Titre
  • 00:42Erythrocyte Isolation from Whole Blood
  • 02:19Treatment of Erythrocytes with Ionomycin and Measurement of Hemolysis
  • 04:43Annexin-V Binding Assay
  • 05:48Flow Cytometry
  • 06:47Results: Flow Cytometry and Confocal Fluorescence Microscopy
  • 08:12Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.

Tags

EryptosisRed Blood CellsCalcium IonophoreIonomycinRinger SolutionGlucose-free BufferHematocritCell SuspensionCentrifugationIncubation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

A “Dual-Addition” Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Read Article