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Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
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Biochemistry
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JoVE Journal Biochemistry
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

English

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8,551 Views

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09:15 min

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January 21, 2020

DOI:

10.3791/60659-v

DOI:
10.3791/60659-v

•

09:15 min
January 21, 2020

•

5 Views
,
1Biomedical Engineering Program,Ohio University, 2Department of Chemical and Biomolecular Engineering,Ohio University

Summary

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A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.

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Cite this Article

Bigdelou, P., Farnoud, A. M. Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore. J. Vis. Exp. (155), e60659, doi:10.3791/60659 (2020).

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