È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore
8,551 Views
•
09:15 min
•
January 21, 2020
DOI:
Summary
Automatically generated
A protocol for the induction of eryptosis, programmed cell death in erythrocytes, using the calcium ionophore, ionomycin, is provided. Successful eryptosis is evaluated by monitoring the localization phosphatidylserine in the membrane outer leaflet. Factors affecting the success of the protocol have been examined and optimal conditions provided.
Read Article
Cite this Article
Bigdelou, P., Farnoud, A. M. Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore. J. Vis. Exp. (155), e60659, doi:10.3791/60659 (2020).
Copy