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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Chapters
- 00:05Introduction
- 00:34Yeast Culture and Lipid Extraction
- 03:55Extracted Lipid Separation
- 05:26Mass Spectrometric Analysis and Lipid Class Identification and Quantification
- 06:53Results: Representative Quantitative Analysis of Saccharomyces Cerevisiae Cellular Lipidomes
- 08:19Conclusion
We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.
