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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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生化学
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JoVE Journal 生化学
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

7,048 Views

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08:56 min

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March 08, 2020

DOI:

10.3791/60616-v

DOI:
10.3791/60616-v

•

08:56 min
March 08, 2020

•

3 Views
, , ,
1生物学科,Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

概要

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We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.

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Cite this Article

Mohammad, K., Jiang, H., Hossain, M. I., Titorenko, V. I. Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. J. Vis. Exp. (157), e60616, doi:10.3791/60616 (2020).

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