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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

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08:56 min

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March 08, 2020

DOI:

10.3791/60616-v

DOI:
10.3791/60616-v

•

08:56 min
March 08, 2020

•

3 Views
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1Biyoloji Bölümü,Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

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We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.

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LipidomeSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryLipid ExtractionYeast CultureInternal Lipid StandardsQuantitative Analysis

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Mohammad, K., Jiang, H., Hossain, M. I., Titorenko, V. I. Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. J. Vis. Exp. (157), e60616, doi:10.3791/60616 (2020).

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