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Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Biochemistry
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JoVE Journal Biochemistry
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

English

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7,048 Views

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08:56 min

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March 08, 2020

DOI:

10.3791/60616-v

DOI:
10.3791/60616-v

•

08:56 min
March 08, 2020

•

3 Views
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1Department of Biology,Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

Summary

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We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.

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LipidomeSaccharomyces CerevisiaeLiquid ChromatographyTandem Mass SpectrometryLipid ExtractionYeast CultureInternal Lipid StandardsQuantitative Analysis

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Cite this Article

Mohammad, K., Jiang, H., Hossain, M. I., Titorenko, V. I. Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry. J. Vis. Exp. (157), e60616, doi:10.3791/60616 (2020).

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