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Chapters
- 00:04Title
- 00:29Differential Fluorescent Cell Labeling
- 02:00Cell-Cell Fusion
- 03:03Fused Cell Enrichment
- 04:18Cell-Cell Fusion Immunofluorescent Staining and Imaging
- 05:30Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
- 06:47Conclusion
The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.
