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Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
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Developmental Biology
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JoVE Journal Developmental Biology
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

English

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8,879 Views

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07:30 min

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December 07, 2019

DOI:

10.3791/60550-v

DOI:
10.3791/60550-v

•

07:30 min
December 07, 2019

•

4 Views
,
1Photonics Group, Department of Physics,Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Summary

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The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

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Cite this Article

Mahen, R., Schulte, R. Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function. J. Vis. Exp. (154), e60550, doi:10.3791/60550 (2019).

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