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Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
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Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
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December 07, 2019
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The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.
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Mahen, R., Schulte, R. Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function. J. Vis. Exp. (154), e60550, doi:10.3791/60550 (2019).
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