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Biologie du développement
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Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
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Biologie du développement
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Journal JoVE Biologie du développement
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

DOI:
10.3791/60550-v

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07:30 min

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December 07, 2019

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,
1Photonics Group, Department of Physics,Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Chapitres

  • 00:04Titre
  • 00:29Differential Fluorescent Cell Labeling
  • 02:00Cell-Cell Fusion
  • 03:03Fused Cell Enrichment
  • 04:18Cell-Cell Fusion Immunofluorescent Staining and Imaging
  • 05:30Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
  • 06:47Conclusion

Summary

Traduction automatique

Traduction automatique

The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

Tags

Keywords: Cell-cell FusionGenome EditingCellular StructureCellular FunctionFluorescent LabelingDye LabelingCell CultureCell FusionPolyethylene Glycol (PEG)Cell ImagingOrganelle HomeostasisOrganelle Function

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