Journal
/
Entwicklungsbiologie
/
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

8,885 Views

•

07:30 min

•

December 07, 2019

DOI:

10.3791/60550-v

DOI:
10.3791/60550-v

•

07:30 min
December 07, 2019

•

4 Views
,
1Photonics Group, Department of Physics,Imperial College London, 2The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, 3Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Summary

Automatically generated

The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

Explore More Videos

Cell-cell FusionGenome EditingCellular StructureCellular FunctionFluorescent LabelingDye LabelingCell CultureCell FusionPolyethylene Glycol (PEG)Cell ImagingOrganelle HomeostasisOrganelle Function

Read Article

Cite this Article

Mahen, R., Schulte, R. Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function. J. Vis. Exp. (154), e60550, doi:10.3791/60550 (2019).

Download .ris file

Copy

Share Video

.

Copy