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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
 

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Article DOI: 10.3791/59973-v • 08:52 min • July 26th, 2019
July 26th, 2019 •
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1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine

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요약
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We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.

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Influenza Antigen Microarray Serum Antibodies Virus Subtypes Measurement Antibody Isotypes Blood Sample Volume High-throughput Measurement Antigenic Landscape Influenza Strains Influenza Infection Antigen Microarray Technique Pathogens Antibodies Against Antigen Targets Serum Specimens Infectious Disease Cases Healthy Controls In-person Workshops Procedure Microarray Printer Low Volume Microarray Spotting Pins 384-well Source Plate Nitrocellulose Coated Glass Slide
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