Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
7,863 Views
•
08:52 min
•
July 26, 2019
DOI:
Summary
Automatically generated
We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.
Read Article
Cite this Article
Khan, S., Jain, A., Taghavian, O., Nakajima, R., Jasinskas, A., Supnet, M., Felgner, J., Davies, J., de Assis, R. R., Jan, S., Obiero, J., Strahsburger, E., Pone, E. J., Liang, L., Davies, D. H., Felgner, P. L. Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes. J. Vis. Exp. (149), e59973, doi:10.3791/59973 (2019).
Copy