Journal
/
Immunology and Infection
/
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

English

Automatically Generated

7,863 Views

•

08:52 min

•

July 26, 2019

DOI:

10.3791/59973-v

DOI:
10.3791/59973-v

•

08:52 min
July 26, 2019

•

4 Views
, , , , , , , , , , , , , , ,
1Division of Infectious Diseases, Department of Medicine,University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology,University of California Irvine

Summary

Automatically generated

We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.

Explore More Videos

Influenza Antigen MicroarrayAntibody BreadthVirus SubtypesHigh-throughput MeasurementAntibody CharacterizationMicroarray PrintingAntigen PrintingMicroarray Probing

Read Article

Cite this Article

Khan, S., Jain, A., Taghavian, O., Nakajima, R., Jasinskas, A., Supnet, M., Felgner, J., Davies, J., de Assis, R. R., Jan, S., Obiero, J., Strahsburger, E., Pone, E. J., Liang, L., Davies, D. H., Felgner, P. L. Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes. J. Vis. Exp. (149), e59973, doi:10.3791/59973 (2019).

Download .ris file

Copy

Share Video

.

Copy