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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Capitoli
- 00:04Titolo
- 01:27Antigen Microarray Printing
- 03:01Probe Sera Microarray Incubation
- 04:25Quantum-Dot-Conjugated Secondary Antibody Labeling
- 05:16Antibody Binding Capture
- 06:03Antibody Binding Quantification
- 07:00Results: Representative Influenza Antigen Microarray Analysis
- 08:03Conclusion
We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.
