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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

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08:52 min

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July 26, 2019

DOI:

10.3791/59973-v

DOI:
10.3791/59973-v

•

08:52 min
July 26, 2019

•

4 Views
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1Division of Infectious Diseases, Department of Medicine,University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology,University of California Irvine

Summary

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We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.

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Cite this Article

Khan, S., Jain, A., Taghavian, O., Nakajima, R., Jasinskas, A., Supnet, M., Felgner, J., Davies, J., de Assis, R. R., Jan, S., Obiero, J., Strahsburger, E., Pone, E. J., Liang, L., Davies, D. H., Felgner, P. L. Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes. J. Vis. Exp. (149), e59973, doi:10.3791/59973 (2019).

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