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冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学
JoVE 신문
생물학
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JoVE 신문 생물학
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
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冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学

DOI:
10.3791/59344-v

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09:20 min

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May 14, 2019

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1Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Chapters

  • 00:04Title
  • 01:06Embryo Embedding and Preparation of Cryosections
  • 02:31Immunofluorescence for pH3
  • 04:54Immunohistochemistry for Labeled Dextran
  • 07:50Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
  • 08:21Conclusion

Summary

자동 번역

자동 번역

该方案演示了早期斑马鱼胚胎的冷冻节的连续免疫荧光和免疫组织化学,从而对特定细胞群进行精确的共定位分析。

Tags

ImmunofluorescenceImmunohistochemistryCryosectionsZebrafish EmbryosCo-localizationAntibody CompatibilityCryosectioningCo-expressionEmbryo OrientationCryostatCharged Glass SlidesPBS WashHumid ChamberBarrier Pen

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