This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.
Ferguson, J. L., Shive, H. R. Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos. J. Vis. Exp. (147), e59344, doi:10.3791/59344 (2019).