Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Jordan  L. Ferguson; Heather R. Shive

doi:10.3791/59344

Journal

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Biology

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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

JoVE Journal
Biology

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JoVE Journal Biology

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

13,314 Views

•

09:20 min

•

May 14, 2019

DOI:

10.3791/59344-v

DOI:

10.3791/59344-v

•

09:20 min

May 14, 2019

•

32 Views

Jordan L. Ferguson*, Heather R. Shive*

¹Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Summary

This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

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