Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Jordan  L. Ferguson; Heather R. Shive

doi:10.3791/59344

Journal

/

Biology

/

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

13,251 Views

•

09:20 min

•

May 14, 2019

DOI:

10.3791/59344-v

DOI:

10.3791/59344-v

•

09:20 min

May 14, 2019

•

32 Views

Jordan L. Ferguson*, Heather R. Shive*

¹Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Summary

This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

Explore More Videos

Immunofluorescence Immunohistochemistry Cryosections Zebrafish Embryos Co-localization Antibody Compatibility Cryosectioning Co-expression Embryo Orientation Cryostat Charged Glass Slides PBS Wash Humid Chamber Barrier Pen

Read Article

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Automatically Generated

Summary

Automatically generated

Explore More Videos