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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
Journal JoVE
Biologie
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Journal JoVE Biologie
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

DOI:
10.3791/59344-v

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09:20 min

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May 14, 2019

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1Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Chapitres

  • 00:04Titre
  • 01:06Embryo Embedding and Preparation of Cryosections
  • 02:31Immunofluorescence for pH3
  • 04:54Immunohistochemistry for Labeled Dextran
  • 07:50Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
  • 08:21Conclusion

Summary

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This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

Tags

ImmunofluorescenceImmunohistochemistryCryosectionsZebrafish EmbryosCo-localizationAntibody CompatibilityCryosectioningCo-expressionEmbryo OrientationCryostatCharged Glass SlidesPBS WashHumid ChamberBarrier Pen

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