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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
JoVE Journal
Biologia
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JoVE Journal Biologia
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

13,239 Views

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09:20 min

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May 14, 2019

DOI:

10.3791/59344-v

DOI:
10.3791/59344-v

•

09:20 min
May 14, 2019

•

32 Views
,
1Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Summary

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This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

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ImmunofluorescenceImmunohistochemistryCryosectionsZebrafish EmbryosCo-localizationAntibody CompatibilityCryosectioningCo-expressionEmbryo OrientationCryostatCharged Glass SlidesPBS WashHumid ChamberBarrier Pen

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Cite this Article

Ferguson, J. L., Shive, H. R. Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos. J. Vis. Exp. (147), e59344, doi:10.3791/59344 (2019).

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