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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
Chapters
- 00:04Title
- 01:22Reagent and Cell Preparation for ROS Measurement
- 05:02Assay Preparation
- 05:53Performing the Assay
- 11:28Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking
- 13:00Conclusion
This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.
