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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

DOI:

13:20 min

March 07, 2019

,

  • 00:04標題
  • 01:22Reagent and Cell Preparation for ROS Measurement
  • 05:02Assay Preparation
  • 05:53Performing the Assay
  • 11:28Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking
  • 13:00Conclusion

概要

自動翻訳

This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.

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