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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
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March 07, 2019
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This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.
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Shehat, M. G., Tigno-Aranjuez, J. Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking. J. Vis. Exp. (145), e59167, doi:10.3791/59167 (2019).
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