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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

DOI:
10.3791/59167-v

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13:20 min

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March 07, 2019

•

,
1Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences,University of Central Florida

Bölümler

  • 00:04Başlık
  • 01:22Reagent and Cell Preparation for ROS Measurement
  • 05:02Assay Preparation
  • 05:53Performing the Assay
  • 11:28Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking
  • 13:00Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.

Etiketler

Flow CytometryROS ProductionMacrophagesFc-gamma Receptor CrosslinkingOxidative StressSuperoxideChronic Granulomatous DiseaseAutoimmune DiseasesNeurodegenerative Diseases

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