JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:33Designing the FRET Assay
- 01:12Preparation of Cul1AMC•Rbx1, the FRET Donor Protein
- 04:42Preparation of FlAsHCand1, the FRET Acceptor Protein
- 05:18Testing and Confirmation of the FRET Assay
- 06:08Measuring the Association Rate Constant
- 07:37Measuring the Dissociation Rate Constant of Cul1•Cand1 in the Presence of Skp•F-box Protein
- 08:33Results: Dynamics Of Protein Complexes
- 10:30Conclusion
