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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

7,870 Views

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10:50 min

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March 14, 2019

DOI:

10.3791/59038-v

DOI:

10.3791/59038-v

•

10:50 min

March 14, 2019

•

2 Views

Melaku Garsamo³, Yun Zhou³, Xing Liu³

¹Department of Biochemistry,Purdue University, ²Department of Botany and Plant Pathology,Purdue University, ³Center for Plant Biology,Purdue University

Tags

FRET Protein Complex Kinetics Association Dissociation COL1 CAND1 7-amino-4-methylcoumarin FlASH Cell Lysis Sonication Centrifugation Glutathione Beads Elution Thrombin Buffer A

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