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利用体外荧光共振能量转移研究毫秒时间尺度蛋白质配合物的动力学

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

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利用体外荧光共振能量转移研究毫秒时间尺度蛋白质配合物的动力学

7,898 Views

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10:50 min

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March 14, 2019

DOI:

10.3791/59038-v

DOI:

10.3791/59038-v

•

10:50 min

March 14, 2019

•

15 Views

Melaku Garsamo^1,3, Yun Zhou^2,3, Xing Liu^1,3

¹Department of Biochemistry,Purdue University, ²Department of Botany and Plant Pathology,Purdue University, ³Center for Plant Biology,Purdue University

Summary

蛋白质-蛋白质相互作用对生物系统至关重要, 对结合动力学的研究为蛋白质复合物的动力学和功能提供了见解。我们描述了一种利用荧光共振能量转移和停止流动技术量化蛋白质复合物动力学参数的方法。

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