ジャーナル
/
生化学
/
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

6,798 Views

•

07:46 min

•

October 08, 2018

DOI:

10.3791/58100-v

DOI:
10.3791/58100-v

•

07:46 min
October 08, 2018

•

2 Views
, , , , ,
1生物学科,Ball State University, 2Department of Genome Sciences,University of Washington, 3Department of Molecular, Cellular, and Developmental Biology,University of Michigan

概要

Automatically generated

Here, we describe a protocol for the biochemical characterization of the yeast RNA-modifying enzyme, Mod5, and discuss how this protocol could be applied to other RNA-modifying enzymes.

Explore More Videos

TRNA-Isopentenyl TransferaseIn Vitro AssayRNA ModificationBiochemical CharacterizationRecombinant EnzymesRNA Modifying EnzymesAcrylamide GelUrea GelTBE BufferRNA Isopentenylation AssayDMAPPMod52-mercaptoethanolRNA Precipitation

Read Article

Cite this Article

Chambers, A. E., Richardson, A. E., Read, D. F., Waller, T. J., Bernstein, D. A., Smaldino, P. J. An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity. J. Vis. Exp. (140), e58100, doi:10.3791/58100 (2018).

Download .ris file

Copy

Share Video

.

Copy