Journal
/
유전학
/
一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Please note that all translations are automatically generated. Click here for the English version.

一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性

DOI:
10.3791/57831-v

•

09:34 min

•

October 25, 2018

•

, , ,
1Division of Molecular Hematology, Lund Stem Cell Center,Lund University

Chapters

  • 00:04Title
  • 00:50Lysis Plate Preparation and Cell Sorting
  • 03:35Reverse Transcription and Specific Target Amplification
  • 04:51Preparation of Sample and Assay Plates
  • 07:41Results: Dynamic Characterization of Cell Populations
  • 08:43Conclusion

Summary

자동 번역

자동 번역

大体积基因表达测量在异构细胞种群中云个体细胞差异。在这里, 我们描述了一个关于如何通过花期活化细胞分类 (immunophenotypically) 的单细胞基因表达分析和指数排序的协议, 以描绘异质性和分子不同细胞种群的特征。

Tags

Single-cell AnalysisStem Cell HeterogeneityCancer HeterogeneityImmunophenotypic ProfilingGene Expression AnalysisLineage PotentialFACS SortingSingle-cell PCRCombinatorial Approach

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性

单细胞测序拷贝数改变的检测

CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

Canalostomy 为成人和新生小鼠内耳局部药物传递的外科治疗方法

复制集方法: 一种高通量方法定量测量秀丽线虫寿命

人牙小泡干细胞的分离、鉴定及微 rna 遗传修饰

人骨髓间充质干细胞细胞外微 rna 的分离、测序和分析

非人类灵长类动物造血干细胞和祖细胞的制备及基因修饰

人类胰腺胰岛的单细胞RNA测序与分析

在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰

Read Article