Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
6,510 Views
•
09:34 min
•
October 25, 2018
DOI:
Summary
Automatically generated
Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.
Read Article
Cite this Article
Sommarin, M. N., Warfvinge, R., Safi, F., Karlsson, G. A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations. J. Vis. Exp. (140), e57831, doi:10.3791/57831 (2018).
Copy