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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Chapters
- 00:04Title
- 00:50Lysis Plate Preparation and Cell Sorting
- 03:35Reverse Transcription and Specific Target Amplification
- 04:51Preparation of Sample and Assay Plates
- 07:41Results: Dynamic Characterization of Cell Populations
- 08:43Conclusion
Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.
