Journal
/
Genetik
/
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

DOI:
10.3791/57831-v

•

09:34 min

•

October 25, 2018

•

, , ,
1Division of Molecular Hematology, Lund Stem Cell Center,Lund University

Kapitel

  • 00:04Titel
  • 00:50Lysis Plate Preparation and Cell Sorting
  • 03:35Reverse Transcription and Specific Target Amplification
  • 04:51Preparation of Sample and Assay Plates
  • 07:41Results: Dynamic Characterization of Cell Populations
  • 08:43Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.

Tags

Single-cell AnalysisStem Cell HeterogeneityCancer HeterogeneityImmunophenotypic ProfilingGene Expression AnalysisLineage PotentialFACS SortingSingle-cell PCRCombinatorial Approach

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Detection of Copy Number Alterations Using Single Cell Sequencing

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Read Article