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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
Chapters
- 00:04Title
- 01:01Mouse Acute Septic Lung Injury
- 02:05Exosome Isolation and Purification
- 03:41Exosome Recipient Uptake and Interaction Validation
- 05:30Results: BALF-isolated Exosome Characterization
- 06:53Conclusion
Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.
