Journal
/
Genetics
/
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

English

Automatically Generated

10,697 Views

•

08:27 min

•

May 21, 2018

DOI:

10.3791/57737-v

DOI:
10.3791/57737-v

•

08:27 min
May 21, 2018

•

2 Views
, ,
1Department of Veterans Affairs,Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine,Emory University

Summary

Automatically generated

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

Explore More Videos

Bronchoalveolar LavageExosomesLipopolysaccharideSeptic Lung InjuryAcute Lung InjuryAcute Respiratory Distress SyndromeAlveolar MicroenvironmentExosome IsolationCentrifugationUltracentrifugationSyringe FiltrationExosome Characterization

Read Article

Cite this Article

Yuan, Z., Bedi, B., Sadikot, R. T. Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury. J. Vis. Exp. (135), e57737, doi:10.3791/57737 (2018).

Download .ris file

Copy

Share Video

.

Copy