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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
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May 21, 2018
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Summary
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Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.
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Yuan, Z., Bedi, B., Sadikot, R. T. Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury. J. Vis. Exp. (135), e57737, doi:10.3791/57737 (2018).
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