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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
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JoVE Journal Genetik
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

10,697 Views

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08:27 min

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May 21, 2018

DOI:

10.3791/57737-v

DOI:
10.3791/57737-v

•

08:27 min
May 21, 2018

•

2 Views
, ,
1Department of Veterans Affairs,Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine,Emory University

Summary

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Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

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Bronchoalveolar LavageExosomesLipopolysaccharideSeptic Lung InjuryAcute Lung InjuryAcute Respiratory Distress SyndromeAlveolar MicroenvironmentExosome IsolationCentrifugationUltracentrifugationSyringe FiltrationExosome Characterization

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Cite this Article

Yuan, Z., Bedi, B., Sadikot, R. T. Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury. J. Vis. Exp. (135), e57737, doi:10.3791/57737 (2018).

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